Searching Genomes by Specific Gene
Ref: Holden MT.,Feil E.J., Lindsay JA. et al (2004) Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance. Proc Natl Acad Sci U S A. 2004 Jun 29;101(26):9786-91.
The sequences of a recent hospital-acquired representative
of the epidemic methicillin-resistant S.aureus EMRSA-16
clone (MRSA252) and a representative of an invasive community-acquired
methicillin susceptible S.aureus clone (MSSA476) have recently
been deposited at Genbank allowing comparative genomics to be applied to
these and other S.aureus strains whose genome sequences are publicly
available. This is illustrated by the comparison of the MSSA strain closely with the MRSA strain.
Here we demonstrate the stepwise fashion that can
be used through WebACT to view the Staphylococcal
chromosomal cassette (SCC) mec-like element described
in the above paper in the MSSA strain that is integrated
at the same site on the chromosome as SCCmec elements in
MRSA strains, but encodes a putative fusidic acid resistance protein
rather than the mec genes which encode the methicillin
The stages involved in viewing this comparison are as follows:
1) choose genomes of interest
2) choose gene of interest and region flanking gene
3) View using ACT
4) Save comparison for distribution to colleagues.